Fig. 7
From: Full L1-regularized Traction Force Microscopy over whole cells
Example of traction recovery on a real cell. a CHO cell expressing Lifeact-GFP; b Pseudo-color image showing the fluorescent beads at the hydrogel surface. The beads of the unstressed and stressed hydrogels have been superposed and pseudo-colored in red and green, respectively; therefore, beads are colored yellow when not displaced. The contrast of the pseudo-color image has been modified to highlight the areas with bead displacements; c Magnitude (in μm) and direction (arrows) of in-plane displacements estimated from the bead images. Recovered traction magnitude (in Pa) and direction (arrows) using: d Tikhonov regularization; e L1-norm regularization; f full L1-norm regularization. The outline of the mask used for traction recovery is shown in red (a) and white (c-f). The scale bar represents 30 μm