Fig. 3From: High-throughput PCR assay design for targeted resequencing using primerXLTargeted resequencing assay statistics. Graphical representation of the targeted resequencing assay statistics for five genes (SACS, SETX, APTX, ANO10, CYP27A1) designed using three primer design tools (primerXL, Optimus Primer and Illumina DesignStudio): a Target coverage efficiency, b Distribution of the amplicon sequence and percentage of amplicons harbouring SNPs (c) or secondary structures d in primer annealing sitesBack to article page