Table 2 Projects statistics overview
From: High-throughput PCR assay design for targeted resequencing using primerXL
Amplicon design | PCR | Sequencing | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Target size | # amplicons | Total amplicon sequence | Exonic (on-target) | Intronic (on-target) | Intronic (near-target) | F-primer | R primer | Assays tested | Failed (No amplification) | Multiple bands | Assays sequenced | No reads | % bp within 2 × mean coverage | |
Project 1 | 83 Kb | 625 | 185 Kb | 108 Kb (58.1%) | 22 Kb (11.7%) | 56 Kb (30.2%) | 13 Kb | 13 Kb | 626 | 17 (2.7%) | NA | 569 | 8 (1.4%) | 70% |
Project 2 | 157 Kb | 723 | 281 Kb | 167 Kb (59.3%) | 27 Kb (9.7%) | 87 Kb (31.0%) | 15 Kb | 15 Kb | 638 | 6 (0.9%) | 79 (12.4%) | NA | NA | NA |
Project 3 | 67 Kb | 375 | 137 Kb | 77 Kb (56.2%) | 14 Kb (9.9%) | 47 Kb (33.9%) | 8 Kb | 8 Kb | 375 | 0 (0.0%) | NA | 375 | 18 (5%) | 59% |
Project 4 | 98 Kb | 625 | 182 Kb | 102 Kb (56.1%) | 29 Kb (15.7%) | 51 Kb (28.2%) | 13 Kb | 13 Kb | 625 | 8 (1.3%) | NA | NA | NA | NA |