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Table 2 Projects statistics overview

From: High-throughput PCR assay design for targeted resequencing using primerXL

   Amplicon design PCR Sequencing
  Target size # amplicons Total amplicon sequence Exonic (on-target) Intronic (on-target) Intronic (near-target) F-primer R primer Assays tested Failed (No amplification) Multiple bands Assays sequenced No reads % bp within 2 × mean coverage
Project 1 83 Kb 625 185 Kb 108 Kb (58.1%) 22 Kb (11.7%) 56 Kb (30.2%) 13 Kb 13 Kb 626 17 (2.7%) NA 569 8 (1.4%) 70%
Project 2 157 Kb 723 281 Kb 167 Kb (59.3%) 27 Kb (9.7%) 87 Kb (31.0%) 15 Kb 15 Kb 638 6 (0.9%) 79 (12.4%) NA NA NA
Project 3 67 Kb 375 137 Kb 77 Kb (56.2%) 14 Kb (9.9%) 47 Kb (33.9%) 8 Kb 8 Kb 375 0 (0.0%) NA 375 18 (5%) 59%
Project 4 98 Kb 625 182 Kb 102 Kb (56.1%) 29 Kb (15.7%) 51 Kb (28.2%) 13 Kb 13 Kb 625 8 (1.3%) NA NA NA NA
  1. Details of the different projects showing the design target coverage, success rate when testing the assays on (q)PCR and sequence coverage