Fig. 4From: Segmentation and classification of two-channel C. elegans nucleus-labeled fluorescence imagesThe process of nuclear segmentation methods. a The raw green-channel image. b The raw red-channel image. c The fused image of (a) and (b). d The binary image after thresholding. e The distance map of (d) (lighter color indicates higher value). f The fused image with seeds. g The binary image after seed-based cluster splitting (too small and dark nuclear regions are excluded). h Final result of the nuclear segmentation with white nuclear boundariesBack to article page