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Fig. 4 | BMC Bioinformatics

Fig. 4

From: Segmentation and classification of two-channel C. elegans nucleus-labeled fluorescence images

Fig. 4

The process of nuclear segmentation methods. a The raw green-channel image. b The raw red-channel image. c The fused image of (a) and (b). d The binary image after thresholding. e The distance map of (d) (lighter color indicates higher value). f The fused image with seeds. g The binary image after seed-based cluster splitting (too small and dark nuclear regions are excluded). h Final result of the nuclear segmentation with white nuclear boundaries

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