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Fig. 2 | BMC Bioinformatics

Fig. 2

From: SimBA: A methodology and tools for evaluating the performance of RNA-Seq bioinformatic pipelines

Fig. 2

SimCT method. SimCT uses a reference FASTA and GTF annotations as input. A first process is intended to introduced biological variations in this reference to create a mutated reference. This new reference is then transfered to FluxSimulator, in order to generate an RNA-Seq experiment. Finaly FluxSimulator output are post-processed to transfer the coordinates from the mutated genome to the original reference

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