Fig. 4

ADAGE signature analysis for the example dataset. a A volcano plot showing both the activity difference and the statistical test significance in the activation test between ∆anr mutant and wild-type. Signatures that lie on the top 10 layers of Pareto fronts are labeled and highlighted in red. The red dotted line indicates the significance of –log10(0.05). b A heatmap showing the absolute activity difference of signatures detected in panel a c A non-symmetric heatmap reflecting the marginal activation significance (adjusted p value in –log10 scale) between every signature pair in panel a For two signatures, one in the row and the other in the column, the heatmap color depicts how strongly the signature in the row is differentially activated after the genes it shares with the signature in the column have been depleted. A cross sign indicates a non-significant activation (adjusted p-value > 0.05). The diagonal shows the activation significance of a signature without overlapping gene removal. d A symmetric heatmap reflecting signature similarity between every signature pair in panel a. The heat color represents the odds ratio that two signatures overlap in their gene contents. Because comparing a signature with itself would result in an odds ratio of infinity, values on the diagonal are replaced by the highest odds ratio among all signature pairs