Fig. 1

The motivation for, outlines of, and action effects of GFF3sort. a An example of incorrectly sorted GFF3 data and how this is rendered in JBrowse. Blocks with the same start position are marked in blue-yellow stripes. The two lines (mRNA) marked in red were placed after their sub-features (exon or UTR). Such an ordering leads to losing the first exon in JBrowse rendering results. See Additional file 2 for the full annotation lines. b Overview of GFF3sort. c An example of features sorted by GFF3sort and this is correctly rendered by JBrowse. In this example, the two lines (mRNA) marked in red are now placed before their sub-features, allowing JBrowse to render them correctly