Fig. 1From: A common base method for analysis of qPCR data and the application of simple blocking in qPCR experimentsOrigin of the Efficiency (E) and C q values. Δ\( {C}_q^{(w)} \) values are derived from the arithmetic means of the technical replicates. Inset A shows the derivation of sample types A and B in an unpaired sample test where sample types derive from different biological replicates. Inset B shows the derivation of sample types A and B in a paired sample test where sample types derive from the same biological replicate. Please note that each E value is logtransformed and multiplied by C q as discussed in the text. This transformation is not shown in the interest of saving spaceBack to article page