Table 3 Analysis of variance (ANOVA) with a blocking factor. Hypothetical data are used to demonstrate an ANOVA for four individuals serving as the replicates spread across two qPCR plates. The qPCR plates serve as a statistical blocking factor. * = expression ratio significantly different from 1
Biological replicate | Group A \( {\Delta C}_{q;r,A}^{(w)} \) | Group B \( {\Delta C}_{q;r,B}^{(w)} \) | Group C \( {\Delta C}_{q;r,C}^{(w)} \) | qPCR plate | Bonferroni-adjusted P-value |
|---|---|---|---|---|---|
r = 1 | 0.855 | 1.203 | 0.866 | 1 | |
r = 2 | 0.711 | 1.056 | 0.799 | 1 | |
r = 3 | 0.582 | 0.890 | 0.522 | 2 | |
r = 4 | 0.699 | 0.775 | 0.669 | 2 | |
Source | Df | MS | F | P | |
Group | 2 | 0.096 | 12.864 | 0.003 | |
Plate Blocking | 1 | 0.153 | 20.487 | 0.002 | |
Error | 8 | 0.007 | |||
Post-hoc testing | Mean Difference \( {\Delta \Delta C}_q^{(w)} \) | 95% C.I. for \( {\Delta \Delta C}_q^{(w)} \) | Expression Ratio \( {10}^{-\Delta \Delta {C}_q^{(w)}} \) | 95% C.I. for \( {10}^{-\Delta \Delta {C}_q^{(w)}} \) | |
Group A vs. B | −0.269 | (−0.128, −0.410) | 1.86 | (1.34, 2.57) | 0.007* |
Group A vs. C | −0.002 | (0.139, −0.143) | 1.00 | (0.726, 1.39) | 1.000 |
Group B vs. C | 0.267 | (0.408, 0.126) | 0.54 | (0.391, 0.748) | 0.007* |