Table 4 Results of paired t-test. A paired t-test and 95% confidence interval are calculated in SPSS using the hypothetical data from Table 1 and three other hypothetical plate experiments. The P-value is from a two-tailed test assuming a mean difference of 0
Biological replicate r | Sample A \( {\Delta C}_{q;r,A}^{(w)} \) | Sample B \( {\Delta C}_{q;r,B}^{(w)} \) | \( {\Delta \Delta C}_{q;r}^{(w)} \) |
|---|---|---|---|
r = 1 | 1.1387 | 1.4077 | −0.269 |
r = 2 | 0.845 | 1.291 | −0.446 |
r = 3 | 0.499 | 1.496 | −0.997 |
r = 4 | 0.699 | 1.172 | −0.473 |
Mean \( {\Delta \Delta C}_q^{(w)} \) | −0.546 | ||
SD for \( {\Delta \Delta C}_q^{(w)} \) | 0.314 | ||
N | 4 | ||
T for \( {\Delta \Delta C}_q^{(w)} \) | −3.48 | ||
P | 0.040 | ||
95% CI for \( {\Delta \Delta C}_q^{(w)} \) | (−1.046, −0.047) | ||
Expression Ratio \( {10}^{-\Delta \Delta {C}_q^{(w)}} \) | 3.52 | ||
95% CI for \( {10}^{-\Delta \Delta {C}_q^{(w)}} \) | (1.11, 11.12) |