Table 5 Analysis of variance (ANOVA) with a blocking factor. Hypothetical data are used to demonstrate an ANOVA with three groups and four individuals serving as the replicates. The groups in this case are paired within individuals and so the individual serves as a statistical blocking factor. * = expression ratio significantly different from 1
Biological replicate | Sample type A \( {\Delta C}_{q;r,A}^{(w)} \) | Sample type B \( {\Delta C}_{q;r,B}^{(w)} \) | Sample type C \( {\Delta C}_{q;r,C}^{(w)} \) | Bonferroni-adjusted P-value | |
|---|---|---|---|---|---|
r = 1 | 0.855 | 1.408 | 0.866 | ||
r = 2 | 0.845 | 1.056 | 0.799 | ||
r = 3 | 0.499 | 1.291 | 0.532 | ||
r = 4 | 0.699 | 1.172 | 0.707 | ||
Source | df | MS | F | P | |
Sample type | 2 | 0.342 | 20.222 | 0.002 | |
Block | 3 | 0.038 | 2.237 | 0.184 | |
Error | 6 | 0.017 | |||
Post-hoc testing | Mean difference \( {\Delta \Delta C}_q^{(w)} \) | 95% C.I. for \( {\Delta \Delta C}_q^{(w)} \) | Expression ratio \( {10}^{-\Delta \Delta {C}_q^{(w)}} \) | 95% C.I. for \( {10}^{-\Delta \Delta {C}_q^{(w)}} \) | |
Sample type A vs. B | −0.507 | (−0.282, −0.732) | 3.21 | (1.91, 5.40) | 0.004* |
Sample type A vs. C | −0.002 | (0.224, −0.227) | 1.00 | (0.60, 1.69) | 1.000 |
Sample type B vs. C | 0.506 | (0.731, 0.281) | 0.31 | (0.19, 0.52) | 0.005* |