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Table 6 Results of improper t-test usage. An improperly implemented paired t-test using hypothetical data from Table 1 and three other hypothetical plate experiments testing the hypothesis of equal gene expression between sample type A and B assuming a mean difference of 0

From: A common base method for analysis of qPCR data and the application of simple blocking in qPCR experiments

Biological replicate r

Sample A \( {\Delta C}_{q;r,A}^{(w)} \)

Sample B \( {\Delta C}_{q;r,B}^{(w)} \)

\( {10^{-\Delta \Delta C}}_{q;r}^{(w)} \) A vs. B

\( {10^{\Delta \Delta C}}_{q;r}^{(w)} \) B vs. A

r = 1

1.1387

1.4077

1.858

0.538

r = 2

0.845

1.291

2.793

0.358

r = 3

0.499

1.496

9.931

0.101

r = 4

0.699

1.172

2.972

0.337

  

Mean

4.39

0.333

  

SD

3.73

0.180

  

N

4

4

  

T

1.82

−7.42

  

P

0.167

0.005