Table 8 Pipeline assembly comparison; Canu assembler is used with MHAP and HISEA as read aligners

E.coli

Contig #

7

3

8

1

NG50

2,771,323

3,969,196

1,223,211

4,642,165

Max contig

2,771,323

3,969,196

1,525,215

4,642,165

% Ref

99.85

99.97

99.82

100.00

Avg idy

99.97

99.99

99.97

99.99

Breakpoints

3

3

3

3

S.cerevisiae

Contig #

43

31

35

29

NG50

540,299

687,498

682,168

774,485

Max contig

964,505

1,534,125

1,537,586

1,534,133

% Ref

98.90

99.35

99.12

99.58

Avg idy

99.81

99.88

99.82

99.88

Breakpoints

17

14

17

14

C.elegans

Contig #

393

170

127

133

NG50

636,401

1,987,017

2,140,282

2,032,954

Max contig

2,648,207

4,224,025

4,227,561

5,669,072

% Ref

96.00

99.84

99.81

99.80

Avg idy

99.76

99.91

99.85

99.91

Breakpoints

431

423

390

435

A.thaliana

Contig #

159

99

140

122

NG50

3,331,858

6,715,370

5,069,662

8,124,422

Max contig

12,892,206

14,177,369

12,890,806

15,940,320

% Ref

92.22

92.55

92.37

92.51

Avg idy

99.17

99.22

99.17

99.22

Breakpoints

2,550

2,693

2,680

2,704

D.melanogaster

Contig #

597

390

553

372

NG50

1,933,939

4,983,913

6,417,268

13,672,005

Max contig

8,238,062

17,900,724

17,366,974

25,767,672

% Ref

95.08

98.55

96.47

98.65

Avg idy

99.80

99.89

99.80

99.87

Breakpoints

1,039

1,383

1,254

1,461