Fig. 1

Depletion of CHC results in the formation of aberrant spindles. a Fluorescence microscopy images of untreated HeLa cells and those treated with luciferase-targeting siRNA (negative control) or CHC-targeting siRNA. Cells were stained with anti-α-tubulin and anti-CHC antibodies and DAPI. Scale bar, 5 μm. b CHC-KD in HeLa cells was assessed by western blotting. α-Tubulin was used as a loading control