Skip to main content
Fig. 1 | BMC Bioinformatics

Fig. 1

From: Cell type discovery and representation in the era of high-content single cell phenotyping

Fig. 1

Identification of myeloid cell subtypes using manual gating and directed automated filtering. A gating hierarchy (a series of iterative two-dimensional manual data partitions) has been established by the investigative team in which peripheral blood mononuclear cells (PBMC) are assessed for expression of HLA-DR and CD3, CD3- cells (Population #5) are assessed for expression of CD19 and CD14, CD19- cells (Population #7) are then assessed for expression of HLA-DR and CD16, HLA-DR+ cells (Population #10) are assessed for expression of HLA-DR and CD14, CD14- cells (Population #19) are assessed for expression of CD123 and CD141, CD141- cells (Population #21) are assessed for expression of CD11c and CD123, and CD11c + cells (Population #23) are assessed for expression of CD1c and CD16. Manual gating results are shown in the top panel; directed automated filter results using the DAFi method, a modified version of the FLOCK algorithm [21] are shown in the bottom panel

Back to article page