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Fig. 7 | BMC Bioinformatics

Fig. 7

From: Assessing microscope image focus quality with deep learning

Fig. 7

Prediction of absolute focus quality on an unseen cell type (MCF-7 cells, from BBBC021 dataset [12]) and unseen stain, Tubulin, using our Fiji (ImageJ) [20] plugin with pre-trained TensorFlow model. A composite image montage was assembled using the center 84 × 84 patch from a randomly selected batch of 240 images. The border hues denote predicted defocus levels (red for best focus), while the lightness denotes prediction certainty. Scale bar is 10 μm or 30 pixels, and a gamma of 0.45 was applied for viewing

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