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Table 1 Morbidity and mortality of AG129 mice infected with wild type and synthetic DENV-2 New Guinea C strain

From: Generation and comparative genomics of synthetic dengue viruses

Animals: Male and female AG129 mice Duration of experiment: 28 days
Virus route: Intraperitoneal injection Volume: 0.2 ml
  Toxicity controls Infected, treated
Treatment Virus titer (ge/μg RNA)a PRNT50 titer (GM ± SD)b Alive / total Virus titer (ge/μg RNA) PRNT50 titer (GM ± SD)b
DENV-2 WT 4/8 1.3 ± 1.4* 1.5 ± 0.7
DENV-2 synth 2/8 2.1 ± 0.3** 2.8 ± 0.2***
Vehicle control 0.0 ± 0.0 1.0 ± 0.0
  1. GM geometric mean, SD standard deviation
  2. aVirus titer expressed as virus titer equivalents (estimated by CCID50/ml stock) per μg total RNA
  3. bmean day to death of mice dying prior to 31 dpi. PRNT50–50% plaque reduction neutralization titers
  4. ***p-value < 0.001, **p < 0.01, *p < 0.05, as compared with sham-infected [vehicle] controls