Fig. 2

SpirPep workflow: This workflow is based on the in silico peptide digestion for bioactive peptide discovery. There were three modules: a data collection and pre-processing: the protein sequences were sent to the Protein database; b in silico peptide digestion: protein sequences were digested with the selected enzymes and the miscleavage number and non-redundant digested peptides were removed to classify them into three groups by peptide length (very short, short, and long peptides); and c bioactivity identification and clustering where these peptide groups were compared against the bioactive peptide sequences with the different methods with 100% in both identity and query or subject coverage