Table 4 Output comparison of identified bioactive peptides of genomic proteins of Arthrospira platensis strain C1 from SpirPep by selecting trypsin and thermolysin enzyme with miscleavage allowed up to three miscleavages against SpirPep in-house bioactive peptide and BIOPEP
Selected enzymes | Miscleavage option | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
0 | 1 | 2 | 3 | |||||||||
No. of peptide | No. of bioactive peptidea | No. of peptide | No. of bioactive peptidea | No. of peptide | No. of bioactive peptidea | No. of peptide | No. of bioactive peptidea | |||||
SpirPep | BIOPEP | SpirPep | BIOPEP | SpirPep | BIOPEP | SpirPep | BIOPEP | |||||
Trypsin | 99,905 | 298 (0.3%) | 247 (0.25%) | 135,899 | 48 (0.04%) | 39 (0.03%) | 137,309 | 1 (0%) | 1 (0%) | 133,145 | 0 (0%) | 0 (0%) |
Thermolysin | 115,846 | 302 (0.26%) | 249 (0.21%) | 285,983 | 182 (0.06%) | 140 (0.05%) | 403,052 | 56 (0.01%) | 32 (0.01%) | 449,652 | 15 (0%) | 10 (0%) |