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Fig. 1 | BMC Bioinformatics

Fig. 1

From: A computational approach to identify cellular heterogeneity and tissue-specific gene regulatory networks

Fig. 1

a Tissue-specific transcriptomic profiling. First, the gene expression data is preprocessed and normalized. Then, the gene expression data and gene set data are integrated together. Each KEGG pathway is statistically evaluated using the traditional algorithms GSEA, PGSEA, and the novel HeteroPath algorithm to identify tissue-specific pathways. Next, the tissue-specific gene regulatory networks are constructed by identification of heterogeneous genes and their regulatory transcription factors as determined by motif enrichment analysis using the ENCODE database. b The HeteroPath algorithm for identifying heterogeneous pathways and gene sets. HeteroPath aims to find the pathways/gene sets that are not only differentially expressed from the global median gene expression value but also appear to be responsible for the regulation of distinct cell types

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