Fig. 3

CpG Methylation Load Ideogram Comparing CP and Control Cohorts. Mean differences in CpG methylation scores (∆ML; control minus CP) were used to calculate a summation methylation load score at 1 Mbp intervals. ∆ML is presented as the inside track using a scatter plot to show higher methylation in controls (green), higher methylation in CP (red), and equivalent methylation in both (gray; abs|∆ML| is less than twice the average ∆ML for the whole genome). The particular “hotspots” that appear in chromosomes 9, 18, 19, and 22 could indicate allelic compositional differences and potential gene targets for future functional and validation studies. The chromosomal locations of the top 200 CpG sites are indicated in two rings by tick marks labeled with the gene name (or “NA” if there is no annotation) in which the CpG site is located. Those CpG sites that had significantly higher methylation levels in the controls are in green. Those CpG sites that had significantly higher methylation levels in the CP subjects are in red. The distribution of sites appears skewed toward chromosomes 11 to 22