Fig. 1

The updated PREPACT 3.0 (www.prepact.de) input interface offering several major enhancements. Several new organelle reference editomes have been added as described in the text. Here exemplarily shown is the selection of the 14 angiosperms out of altogether 27 chloroplast editomes now available (a). U-to-C editing and C-to-U editing may now be selected individually, and a new feature allows prognosis of U-to-C reverse editing to remove stop codons even when no conserved arginine or glutamine codons would be restored (b). Editing prediction may be restricted to sites where RNA editing has been identified previously in at least one of the references or to always include such sites in the output (c) even when below the overall commons thresholds defined above. As an alternative to FASTA-formatted input of a query, GenBank database accessions may simply be given as exemplarily shown for the Cucumis sativus cpDNA (d). Overall enhanced handling of the sequence input is particularly relevant for the multiple sequence alignment analysis modes (Additional file 1)