Fig. 1

Differential expression analysis of low variance simulated data. This figure shows the performance (y-axis) of a complete differential expression analysis, organized by differential expression method (x-axis) and the number of replicates per group (panel). The acronyms clr, iqlr, malr, ii1, and ii5 describe log-ratio transformations (see Methods). The acronyms slFMD, slQUASI, and stsl describe alignment and quantification procedures (see Methods). Missing data suggest that the method did not call any transcripts differentially expressed (and therefore has no precision or recall). The horizontal line indicates a precision of 0.95, equivalent to the requested false discovery rate (FDR) of 0.05