Fig. 2
From: BARCOSEL: a tool for selecting an optimal barcode set for high-throughput sequencing
Web interface to BARCOSEL. User needs to give only two inputs: (1) Candidate barcodes in FASTA format and (2) the number of barcodes to be selected. Sequences can imported either by copy-pasting them in a text box or uploading a FASTA file. After user has pressed submit button, the web page is returned containing an optimal set of barcodes and an image showing nucleotide balance in each barcode position. In case no solution can be found, user gets a report. Optional input parameters become available when pressing Advanced button. These include (3) an initial set of barcodes if BARCOSEL is used to augment an existing set, (4) distance type (Hamming or Levenshtein) and minimum barcode distance required (default is 3 tolerating one sequencing error), and (5) parameters related to lpsolve: maximum computation time (default is 10 seconds), branch-and-bound search depth (0 means no restrictions), and basis crash parameter related to initialization. There is no need to change lpsolve related parameters unless no acceptable solution is found with default values