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Fig. 4 | BMC Bioinformatics

Fig. 4

From: A novel computational method for automatic segmentation, quantification and comparative analysis of immunohistochemically labeled tissue sections

Fig. 4

Selection of training pixels. a sample tissue images showing CD3 marker stained with brownish color (a), and CD163 marker stained in reddish color (b and c). In the images we show examples of manually selected marker-pixels (green arrows), rectangular areas containing obvious not-marker pixels (black rectangular areas), and critical not-marker pixels (black arrows)

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