Skip to main content
Fig. 6 | BMC Bioinformatics

Fig. 6

From: A novel computational method for automatic segmentation, quantification and comparative analysis of immunohistochemically labeled tissue sections

Fig. 6

Marker co-existence analysis from serial tissue sections. Top row: metastatic melanoma tissue labelled with CD3 (a), CD8 (b), and CD163 (c) markers stained in reddish color. d overlapped tissue shapes before shape-based registration; e and overlapped tissue shapes after registration. Note that in d the tissue shapes are not aligned; after image registration the tissue shapes are aligned and the co-existing markers are overlapped (as shown in detail g). Yellow colors appear when red and green markers are overlapped, purple colors appear when red and blue markers are overlapped, white colors appear when all the three markers are overlapped. f overlapped concentration regions after registration; g zoomed detail of overlapped CD3 and CD8 markers; h zoomed detail of overlapped CD3 and CD8 concentration regions. The GTRO computed on these images before registration equals 76.5%, while it increases to 97.9% after registration

Back to article page

BMC Bioinformatics

ISSN: 1471-2105

Contact us