Fig. 2

Checking the specificity of circRNA primers. a Checking the specificity of divergent primers for has_circ_0020707, a circRNA derived from RPLP2. Length, polymerase chain reaction (PCR) product size; F0R, primer characteristic (1, convergent primer; 0, divergent primer; F + No., forward primer spanning the spliced junction [No. represents the base count spanned by the primer]; and R + No., reverse primer spanning the spliced junction). b Checking the specificity of primers, with one primer spanning the spliced junction. c Localization of the divergent primers in has_circ_0020707. d Localization of the divergent primers in has_circ_0020706, which is also derived from RPLP2. e Localization of the primers, with the forward primer spanning the spliced junction of has_circ_0020707