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Fig. 2 | BMC Bioinformatics

Fig. 2

From: Rscreenorm: normalization of CRISPR and siRNA screen data for more reproducible hit selection

Fig. 2

Plate effects before and after rscreenorm. Removal of plate effects per replicate of an arrayed screen by rscreenorm. Boxplots of log2-raw viability values (top) and rscreenorm scores (bottom) for replicate 1 of cell line SW1573. Plates 1-272 (from left to right) are displayed with the same colours for negative controls (left boxes), sample siRNAs (middle boxes) and positive controls (right boxes). Plate 1 is coloured orange (left-most box for each well type), and plate 272 is coloured pink (right-most box). The top graph displays -log2-raw data, to make boxes in both plots easily comparable – so in all cases, values indicating more lethality are displayed higher than those corresponding to more viability. Vertical axis: top graph has -log2-viability; bottom graph has rscreenorm scores (computed using the log2-intensity read-outs)

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