Fig. 2From: Rscreenorm: normalization of CRISPR and siRNA screen data for more reproducible hit selectionPlate effects before and after rscreenorm. Removal of plate effects per replicate of an arrayed screen by rscreenorm. Boxplots of log2-raw viability values (top) and rscreenorm scores (bottom) for replicate 1 of cell line SW1573. Plates 1-272 (from left to right) are displayed with the same colours for negative controls (left boxes), sample siRNAs (middle boxes) and positive controls (right boxes). Plate 1 is coloured orange (left-most box for each well type), and plate 272 is coloured pink (right-most box). The top graph displays -log2-raw data, to make boxes in both plots easily comparable – so in all cases, values indicating more lethality are displayed higher than those corresponding to more viability. Vertical axis: top graph has -log2-viability; bottom graph has rscreenorm scores (computed using the log2-intensity read-outs)Back to article page