Fig. 1
From: PIPEBAR and OverlapPER: tools for a fast and accurate DNA barcoding analysis and paired-end assembly
PIPEBAR workflow. a, b Conversion of chromatograms files to FASTQ files. c, ) Trimming and filtering of the FASTQ files for low quality bases based on Phred quality. e Overlapping paired-end reads considering both substitution and indel errors by OverlapPER. f report file of barcode sequences produced given a PHRED quality value as parameter. g Fasta files for the merged reads and FASTQ files for the not-merged reads. h In case of analyzing a coding region, PIPEBAR applies stop codon and frameshift correction