Fig. 2
From: PIPEBAR and OverlapPER: tools for a fast and accurate DNA barcoding analysis and paired-end assembly
Stop-codon and frameshift corrections. PIPEBAR translates the sequence in 3 forward and 3 reverse frames, selects the frame where the impact of the found stop codons is minimum. Identifying the best translation frame, the stop codons located in the extremities of the sequence are trimmed, generating at the end of the process a sequence that is ready to be submitted to NCBI and BOLD databases