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Table 3 Analysis of PIPEBAR’s barcodes with respect to the mean similarity percentage of all the generated sequences to its respective Geneious’ sequences, mean length of the alignment, total sum of mismatches and gap openings by applying Blastn against the Geneious’ reference sequences

From: PIPEBAR and OverlapPER: tools for a fast and accurate DNA barcoding analysis and paired-end assembly

 

Mean % identity

Mean length (bp)

Mismatches

Gap openings

Dataset 1

99.9 ± 0.08

545.74 ± 105.95

191

45

Dataset 2

99.97 ± 0.03

669.43 ± 19. 26

59

21

Dataset 3

99.93 ± 0. 12

596.72 ± 93.72

57

42