Fig. 6

Sequence alignments of spanin sharing homology across different architectures. a, d, g are pictorial representations of the homologous regions of DLF12 and EE36P1, T5 and Shivani. and Jersey and FSL_SP-031 respectively. b, c show sequence alignments of periplasmic domains of i-spanin and o-spanin of DLF12 and EE36P1 respectively, (e, f) show sequence alignments of periplasmic domains of i-spanin and o-spanin of T5 and Shivani respectively and (h, i) show sequence alignments of periplasmic domains of i-spanin and o-spanin of Jersey and FSL_SP-031 respectively. We chose to include the full-length sequences in the alignments as the shared DNA region codes for the signal sequence of the o-spanin. Blue and yellow rectangles represent i-spanin and o-spanin respectively, whereas the pink and orange rectangles represent the approximate position of the N-terminal TMD sequence in the i-spanin and the N-terminal lipoylation signal sequence in the o-spanin respectively. Grey shaded region indicates the region of sequence similarity between homologs. Gene names are in bold, all genes are drawn to scale, and their position in the genome in terms of kilobases is denoted by the numbers below the line. Conservation at different positions is shown below with colors (Red indicates maximum conservation and blue indicates least conservation). A scale indicating the relative position of the residues and the approximate size of various domains is shown above the alignments