Fig. 1
From: XenofilteR: computational deconvolution of mouse and human reads in tumor xenograft sequence data
Overview of XenofilteR workflow. Sequence reads (fastq) from PDX are mapped with the appropriate aligner (e.g. BWA, Tophat, STAR) to both a human and mouse reference genome. Sequence reads that only map to a single reference genome are classified to that specific organism. For seqeunce reads that map to both the human and mouse reference genome the edit distance is calculated which is defined by the number of base pairs different between the sequence read and the reference genome. Next, XenofilteR classifies the sequence reads as ‘human’ or ‘mouse’ based on the edit distance