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Table 1 Example of the mapping from a pair of degenerate primers to a primer-set-pair

From: Optimizing PCR primers targeting the bacterial 16S ribosomal RNA gene

DG (forward)

DG (reverse)

NDG (forward)

NDG (reverse)

ACGTHACGT

RACGTYACGT

ACGTAACGT

AACGTCACGT

 

ACGTCACGT

AACGTTACGT

ACGTTACGT

GACGTCACGT

GACGTTACGT

  1. DG degenerate primer, NDG non-degenerate primer
  2. The degenerate bases are shown in bold
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BMC Bioinformatics

ISSN: 1471-2105

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