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Fig. 5 | BMC Bioinformatics

Fig. 5

From: Expression analysis of RNA sequencing data from human neural and glial cell lines depends on technical replication and normalization methods

Fig. 5

Conditional (cell line) and technical (library, lot, flow cell) contributions to variation between replicate samples. Box plots (a, c, e, g) show log2 fold-changes between technical replicates for both cell lines (hNSC, green; NHA, violet) and between cell lines (blue). Pie charts demonstrate that the contribution of different components to sample variance (b, d, f, h). Principal variance component analysis revealed the influence of cell line (blue), flow cell (red), library preparation (yellow), cell lot (light green), and re-sidual variance (black). The estimates are shown for log2 transformed data (a, b), RPKM normalized data (c, d), TMM normalized data (E,F) and UQS normalized data (G, H). Adapted by permission from Springer [22], Copyright 2017

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