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Table 2 Experimental parameters contributing to variation in the study were assessed by analysis of eight forward and eight reverse RNA-Seq data files from each cell line (32 data files total)

From: Expression analysis of RNA sequencing data from human neural and glial cell lines depends on technical replication and normalization methods

Component Number of Replicates Type of Variation Effect
Cell line 2 (NHA, hNSC) Conditional Fixed
Lot 2 per cell line Technical Random
Library preparation 2 per lot Technical Random
Flow Cell 2 per library Technical Random