Table 2 Experimental parameters contributing to variation in the study were assessed by analysis of eight forward and eight reverse RNA-Seq data files from each cell line (32 data files total)
Component | Number of Replicates | Type of Variation | Effect |
|---|---|---|---|
Cell line | 2 (NHA, hNSC) | Conditional | Fixed |
Lot | 2 per cell line | Technical | Random |
Library preparation | 2 per lot | Technical | Random |
Flow Cell | 2 per library | Technical | Random |