Fig. 1

Excerpt of Eukaryotic Sedolisin Sequence Alignment. The demonstrated sequences are representatives of the three mayor phylogenetic clusters, as indicated by the tree placed at the end of the alignment, extracted from the complete MSA (Additional file 5). 3EE6, ngr0005053 and sal0001152 are non-fungal sequences; SED_A and Aorsin are fungal endosedolisins; SED_B and SED_C are fungal TPPs. Oma0001582 and Asa0002533 are additional fungal sequences with unknown characteristics. Horizontal arrows and helices indicate sheet and helix regions, respectively. Nomenclature of secondary structure elements and numbers according to 3EE6. The vertical arrows represent beginning and end of the peptide linker. Sequence in gray box represents the SED_A inserts (numbers according to SED_A sequence): CSI1 (190–217), CSI2 (345–371), CSI3 (427–435) and CSI4 (513–525). Black stars indicate catalytic residues E, D, and S whereas the gray star indicates the oxyanion D. Connected boxes, numbered 1, 2 and 3 indicate disulfide bridges identified in the 3EE6 structure. F1 points to two cysteines strictly conserved among all fungal sedolisins but absent in non-fungal sedolisins. Red shading (identity) and fonts (similarity) highlight conserved positions