Table 2 The assembly contiguity (scaffold NG50 and NGA50) and correctness (number of misassemblies) metrics with and without correction using Tigmint prior to scaffolding with ARCS
From: Tigmint: correcting assembly errors using linked reads from large molecules
Sample | Assembly | NG50 (Mbp) | NGA50 (Mbp) | Misass. | Reduction |
|---|---|---|---|---|---|
HG004 | ABySS | 3.65 | 3.09 | 790 | NA |
| Â | ABySS+Tigmint | 3.47 | 3.09 | 574 | 216 (27.3%) |
| Â | ABySS+ARCS | 9.91 | 7.86 | 823 | NA |
| Â | ABySS+Tigmint+ARCS | 26.39 | 16.43 | 641 | 182 (22.1%) |
HG004 | DISCO+ABySS | 10.55 | 9.04 | 701 | NA |
| Â | DISCO+ABySS+Tigmint | 10.16 | 9.04 | 666 | 35 (5.0%) |
| Â | DISCO+ABySS+ARCS | 29.20 | 17.05 | 829 | NA |
| Â | DISCO+ABySS+Tigmint+ARCS | 35.31 | 23.68 | 804 | 25 (3.0%) |
HG004 | DISCO+BESST | 7.01 | 6.14 | 568 | NA |
| Â | DISCO+BESST+Tigmint | 6.77 | 6.14 | 493 | 75 (13.2%) |
| Â | DISCO+BESST+ARCS | 27.64 | 15.14 | 672 | NA |
| Â | DISCO+BESST+Tigmint+ARCS | 33.43 | 19.40 | 603 | 69 (10.3%) |
HG004 | Supernova | 38.48 | 12.65 | 1005 | NA |
| Â | Supernova+Tigmint | 17.72 | 11.43 | 923 | 82 (8.2%) |
| Â | Supernova+ARCS | 39.63 | 13.24 | 1052 | NA |
| Â | Supernova+Tigmint+ARCS | 27.35 | 12.60 | 998 | 54 (5.1%) |
HG004 | Falcon | 4.56 | 4.21 | 3640 | NA |
| Â | Falcon+Tigmint | 4.45 | 4.21 | 3444 | 196 (5.4%) |
| Â | Falcon+ARCS | 18.14 | 9.71 | 3,801 | NA |
| Â | Falcon+Tigmint+ARCS | 22.52 | 11.97 | 3,574 | 227 (6.0%) |
NA12878 | Canu | 7.06 | 5.40 | 1688 | NA |
| Â | Canu+Tigmint | 6.87 | 5.38 | 1600 | 88 (5.2%) |
| Â | Canu+ARCS | 19.70 | 10.12 | 1736 | NA |
| Â | Canu+Tigmint+ARCS | 22.01 | 10.85 | 1,626 | 110 (6.3%) |
Simulated | ABySS | 9.00 | 8.28 | 272 | NA |
| Â | ABySS+Tigmint | 8.61 | 8.28 | 217 | 55 (20.2%) |
| Â | ABySS+ARCS | 23.37 | 17.09 | 365 | NA |
| Â | ABySS+Tigmint+ARCS | 30.24 | 24.98 | 320 | 45 (12.3%) |