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Table 5 Simulated high coverage datasets analysis where the inputs of EIM pipeline are the read set and genome reconstructed by Bowtie2

From: Assessing the impact of exact reads on reducing the error rate of read mapping

Assembly

Exact

EIM (v2)

Bowtie2

ReadSet1

   

Contigs-500

68

2

6

N50 (kbp)

156.4

3543.3

2385.6

Errors

0

29

38

IUPAC-codes

0

11

137

Genome-Fraction (%)

99.381

99.999

100

ReadSet2

   

Contigs-500

142

3

5

N50 (kbp)

62.7

1371.6

939.1

Errors

0

45

92

IUPAC-codes

0

29

246

Genome-Fraction (%)

99.338

100

99.997

ReadSet3

   

Contigs-500

96

6

3

N50 (kbp)

94.4

1096.2

3267.7

Errors

2

54

87

IUPAC-codes

0

11

140

Genome-Fraction (%)

99.285

99.997

100

ReadSet4

   

Contigs-500

77

3

3

N50 (kbp)

115.5

2337.4

1530.2

Errors

6

55

72

IUPAC-codes

0

15

34

Genome-Fraction (%)

99.436

99.998

100

  1. The evaluation metrics has been defined in the text. The columns headed ’Exact’, ’EIM (v2)’ and ’Bowtie2’ represent the contiguity and quality of contigs constructed by ExactMapping step of EIM, EIM (v2) and Bowtie2, respectively