Fig. 4

Comparing the percentage of healthy-like cells with traditional average-based metrics and another multi-dimensional analysis approach [9]. a Each panel depicts one channel (nuclear shape – DAPI channel – is not considered in Ref. [33] and therefore is not included here). Each dot represents a siRNA sample. Horizontal axis shows the average-based metric, and vertical axis shows our percentage-based metric. In general, siRNA samples on the right are more different from progerin-like controls than samples to their left. Solid gray lines represent hit thresholds for corresponding metrics. b Similar to (a), each panel shows one of the three channels in the screen. Each circle is a siRNA sample. The horizontal axis shows the inverse of the distance to healthy-like (GFP-progerin repressed) controls: larger values indicate increased similarity of the siRNA to GFP-progerin repressed controls. The vertical axis shows the percentage of healthy-like cells, and the dashed lines are thresholds for hits in the respective channels