Fig. 6

Typical cells in siRNA perturbations identified as different from both controls. a siPHF13 is an outlier in the progerin channel: cells treated with siPHF13 express more progerin than the progeria-like control cells (f), and the expressed progerin appears to be distributed differently from the progeria control. b siNEDD4 is an outlier in the lamin B1 channel; cells treated by siNEDD4 express more lamin B1 than the healthy-like control cells (c), and the expression is less homogeneous. In addition, the expression of lamin B1 is spatially co-localized with the expression of progerin in siNEDD4-treated cells. d siTRIML1 is an outlier in both DAPI (nuclear shape) and progerin channels. Cells treated by siTRIML1 tend to have elongated nuclei compared to the healthy-like and the progeria-like controls. Also, clusters and increased progerin expression (compared to the progeria-like control (f)) can be observed. e siRNF8 is an outlier in the γH2AX (DNA damage) channel. Note that the contours obtained from the DAPI channel appear slightly smaller and misaligned with the images obtained in the lamin B1 channel (see Additional file 1: Figure S2 for the analysis of cross-channel discrepancies). f Progeria-like control cells. The scale bar is 5 μm