Fig. 5
From: LimeSeg: a coarse-grained lipid membrane simulation for 3D image segmentation
Endoplasmic reticulum (ER) and plasma membrane (PM) segmentation of a FIB-SEM HeLa cell dataset. a - Typical data slice where the nucleus, ER and PM are visible. b - Resulting segmentation of ER (magenta) and PM (green). c - Segmented ER and PM, overlaid on the original data. d - Missed parts of nuclear envelope where the double membrane is too thin to be correctly segmented (left). Spurious hole generated during segmentation (right). e - Detail showing plasma membrane invagination in 2D and 3D. F - Detail of nuclear pore complex as seen on 2D and on 3D. Scalebars: a, c: 1μm; d, e, f: 100nm