Fig. 1

MEDME experiment versus MEDIPS calibration plot. a Calibration plot from the MEDIPS method to estimate CpG density bias. The blue stripes indicate grouped bin reads vs. corresponding bin CpG counts. The relationship between the means of bin reads and bin CpG counts are shown by the red bell-curve. The green line represents fitting a simple linear regression model of the relationship from low CpG density regions. b The sigmoidal relationship between the MeDIP-ChIP enrichment signal and CpG density was revealed by MEDME in log-scale. The red dots signify the median enrichment signal within a 1 k bp window across the dynamic range of numbers of methylated CpGs