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Fig. 1 | BMC Bioinformatics

Fig. 1

From: SMoLR: visualization and analysis of single-molecule localization microscopy data in R

Fig. 1

Workflow for analysis of SMLM data with SMoLR. Workflow across external single-molecule localization software (blue), ImageJ/FIJI (green) and SMoLR (pink). (a) SMLM data is extracted from microscopy images, and represented in table format with as minimum information x and y coordinates, and localization precision. (b) The extracted data is analyzed with SMoLR or other visualization programs as an entire image. (c) Using either ImageJ/FIJI or SMoLR regions of interest are determined and selected, either manually or automatically using selection criteria. (d) The localization data is split by SMoLR into a list containing data of each ROI, these individual ROIs can be analyzed in more detail at once. Resulting parameters can easily be statistically explored using R

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