Fig. 1

Architecture of the Model checking based approach: Model checking based approach requires a formal description of the behavior of the signaling pathways. The differential expression of genes between the conditions under study are used to estimate the parameters of the model or define the initial configuration. Once the model is specified by the proper formal language, it should be converted into discrete time or continues time Markova chains model which is usually done by the chosen model checking tool. After that, the model is given to score calculator which allocates a score to each pathway with the help of a model checking tool. For example, Score computation requests the model checking tool to compute the possibility of a cellular response activation