Fig. 4

Synergy Square and histograms. a Black bars: Distribution of absolute synergy scores (i.e. the number of cell lines that showed synergy) for each tested drug pair. Gray bars: a randomization that conserved the number of synergies per cell line, but reassigned synergic drug pairs within a cell line. The observed distribution has much more 0 scores and high scores than the randomized one. b-e Randomization that conserves the sensitizing property of each drug. b A random absolute synergy score matrix that conserves the total synergy score per drug, in order to conserve each drug sensitizing properties in the randomization (generated with binomials distributions). c The observed absolute synergy score matrix. Drugs are ordered according to their sensitizing potential. d Comparison of the random and observed distributions (e) Number of synergy scores greater than 12, observed (vertical line) versus 1000 randomizations (histogram bars). f Sensitizing potential of each individual drug. For each drug, the number of synergies observed across all second drugs and cell lines, in the high dose assay versus the low dose assay. The number of synergies in common between the low dose and high dose assays was tested for each drug with the fisher test (black dots represent the 77 drugs with significant overlap)