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Fig. 1 | BMC Bioinformatics

Fig. 1

From: RIP-Chip analysis supports different roles for AGO2 and GW182 proteins in recruiting and processing microRNA targets

Fig. 1

RIP-Chip experiments overview. a and c Western Blot analysis of proteins immunoprecipitated and co-immunoprecipitated with anti-AGO2 or anti-GW182 antibody (IP). IgGs in a) are the negative controls. IN and FT made up 1% of the cytoplasmic lysate used for each IP sample. GW182 was specifically co-immunoprecipitated with AGO2 (c, left panel), and AGO2 was specifically co-immunoprecipitated with GW182 (c, right panel). b Enrichment analysis of seven highly expressed miRNAs in anti-AGO2 and anti-GW182 IP compared to IgG-IP controls. d Average Linkage Cluster analysis of mRNA and miRNA expression profiles of IP, IN and FT samples from three independent experiments; distance is computed as 1- Correlation (Pearson). AGO2-IP and GW182-IP mRNA expression profiles are highlighted in blue and green, respectively. In mRNA expression clustering, we considered all the 16,323 genes with a detected expression level in the samples considered. In miRNA expression clustering, we considered 508 miRNAs with a detected expression level in at least one sample

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