Fig. 2
From: GRIBCG: a software for selection of sgRNAs in the design of balancer chromosomes
GRIBCG procedural steps in the generation of top sgRNA lists. Each chromosome is analyzed, selecting all potential 23-bp sequences containing 5′-NGG-3.’ Then, 18-bp partial sequence sites (PSS) are screened across all other chromosomes, producing an average cut distance for a given PTS based on PSSs. Off-target scoring calculated by counting presence of PSSs for given PTS on other chromosomes. PTSs with PSS sites on other chromosomes are rejected. Potential target sequences are then filtered by optimal cut counts for their chromosome. On-target scoring is performed for all remaining PTSs. A final list of top sgRNA designs per chromosome is generated based on off-target, on-target scoring, and coverage. Dashed arrows indicate optional parameters where users may upload a single file containing gene locations