Fig. 4
From: GRIBCG: a software for selection of sgRNAs in the design of balancer chromosomes
Ideal balancer (top) produced by a single sgRNA in fourth chromosome of A. thaliana. Each inversion breakpoint protects the surrounding 4 Mbps from recombination. GRIBCG optimizes coverage, resulting in more evenly spaced breakpoints. A non-ideal balancer (below) produced by a single sgRNA, on the same chromosome as above, where breakpoints are situated near one another leaving most of the chromosome unprotected. Each vertical line represents a given double-stranded break