Fig. 5
From: GRIBCG: a software for selection of sgRNAs in the design of balancer chromosomes
Comparison between GRIBCG results and existing fruit fly balancer chromosomes. a. SM6a is the most common second chromosome balancers in D. melanogaster, as described in Miller et al. 2016. Each vertical line represents a given double-stranded break. These breakpoints span the entire arm, encompassing both intergenic and genic regions. Below, the top ideal sgRNA for the same chromosome is depicted as generated by GRIBCG. b. TM3 and TM6 are the most common third chromosome balancers in D. melanogaster. Arrows indicate the breakpoints corresponding to the known sequence of inversions in the generation of TM3 or TM6. Below, the top ideal sgRNA for the same chromosome is depicted as generated by GRIBCG